Electrochemical Analysis of Chemically Modified DNA at Gold Electrodes

0506690 - BFÚ 2020 RIV CZ eng C - Conference Paper (international conference)
Vidláková, Pavlína - Havran, Luděk - Fojta, Miroslav
Electrochemical Analysis of Chemically Modified DNA at Gold Electrodes.
XXXVI. Moderní elektrochemické metody. Sborník přednášek. Ústí nad Labem: Best servis, 2016 - (Navrátil, T.; Fojta, M.; Schwarzová, K.), Č. 2016 (2016), s. 282-284. ISBN 978-80-905221-4-5.
[Moderní elektrochemické metody /36./. Jetřichovice (CZ), 23.05.2016-27.05.2016]
R&D Projects: GA ČR GA15-08434S; GA ČR GBP206/12/G151
Institutional support: RVO:68081707
Keywords : enzymatic incorporation * nucleic-acids * immobilization
OECD category: Physical chemistry

DNA labeling is used to increase sensitivity of its electrochemical detection. Covalently bound markers based on a complex of osmium tetroxide with 22-bipyridine(Os,bipy) is one of those frequently applied. Os bipy reacts with pyrimidines (mainly thymines) in single stranded regions of DNA, therefore it represents a useful marker for the analysis of DNA structure or labeling of hybridization probes . Another possibility of DNA labeling is the incorporation of chemically modified dNTPs into DNA molecules with using primer extension. DNA labeling in connection with voltammetry at gold electrodes has application potential in development of sensors for studying of DNA structure and interaction.
Permanent Link: http://hdl.handle.net/11104/0297888