Temperature alters susceptibility of Picea abies seedlings to airborne pollutants: The case of CdO nanoparticles

0506604 - ÚVGZ 2020 RIV GB eng J - Journal Article
Večeřová, Kristýna - Večeřa, Zbyněk - Mikuška, Pavel - Coufalík, Pavel - Oravec, Michal - Dočekal, Bohumil - Surá, Kateřina - Veselá, Barbora - Pompeiano, A. - Urban, Otmar
Temperature alters susceptibility of Picea abies seedlings to airborne pollutants: The case of CdO nanoparticles.
Environmental Pollution. Roč. 253, OCT 2019 (2019), s. 646-654. ISSN 0269-7491. E-ISSN 1873-6424
R&D Projects: GA MŠMT(CZ) LO1415; GA MŠMT(CZ) EF16_013/0001609
Research Infrastructure: CzeCOS II - 90061
Institutional support: RVO:86652079 ; RVO:68081715
Keywords : CdO nanoparticles * Primary/secondary metabolites * Metabolomics * Norway spruce * Photosynthesis
OECD category: Analytical chemistry; Analytical chemistry (UIACH-O)
Impact factor: 6.793, year: 2019
Method of publishing: Open access with time embargo
https://reader.elsevier.com/reader/sd/pii/S0269749119303227?token=809AF84CD9D88C571611D638E47320160B12F13EB917228D401467235C8A6A70B42A553C58498A4E3BC8EB8B36B8B6E1

Although plants are often exposed to atmospheric nanoparticles (NPs), the mechanism of NP depositionand their effects on physiology and metabolism, and particularly in combination with other stressors, arenot yet understood. Exploring interactions between stressors is particularly important for understandingplant responses in urban environments where elevated temperatures can be associated with air pollu-tion. Accordingly, 3-year-old spruce seedlings were exposed for 2 weeks to aerial cadmium oxide (CdO)NPs of environmentally relevant size (8e62 nm) and concentration (2 105cm 3). While half theseedlings were initially acclimated to high temperature (35 C) and vapour pressure deficit (VPD,2.81 kPa), the second half of the plants were left under non-stressed conditions (20 C, 0.58 kPa). Atomicabsorption spectrometry was used to determine Cd content in needles, while gas and liquid chroma-tography was used to determine changes in primary and secondary metabolites. Photosynthesis-relatedprocesses were explored with gas-exchange and chlorophyllfluorescence systems. Our work supportsthe hypothesis that atmospheric CdO NPs penetrate into leaves but high temperature and VPD reducesuch penetration due to stomatal closure. The hypothesis that atmospheric CdO NPs influences physi-ological and metabolic processes in plants was also confirmed. This impact strengthens with increasingtime of exposure. Finally, we found evidence that plants acclimated to stress conditions have differentsensitivity to CdO NPs compared to plants not so acclimated. Thesefindings have important conse-quences for understanding impacts of global warming on plants and indicates that although the effects ofelevated temperatures can be deleterious, this may limit other forms of plant stress associated with airpollution.
Permanent Link: http://hdl.handle.net/11104/0297821