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Analysis of Products of Nontemplate Enzymatic Synthesis of DNA Oligonucleotides using Voltammetric Methods
- 1.0506559 - BFÚ 2020 RIV CZ eng C - Conference Paper (international conference)
Hermanová, Monika - Havranová-Vidláková, Pavlína - Ondráčková, Anna - Fojta, Miroslav
Analysis of Products of Nontemplate Enzymatic Synthesis of DNA Oligonucleotides using Voltammetric Methods.
XXXVIII. Moderní elektrochemické metody. Sborník přednášek. Ústí nad Labem: Best Servis, 2018 - (Navrátil, T.; Fojta, M.; Schwarzová, K.), Č. 2018 (2018), s. 77-80. ISBN 978-80-905221-6-9.
[Moderní elektrochemické metody /38./. Jetřichovice (CZ), 21.05.2018-25.05.2018]
R&D Projects: GA ČR GBP206/12/G151; GA MŠMT EF15_003/0000477
Institutional support: RVO:68081707
Keywords : Nontemplete DNA synthesis * Hanging mercury drop electrode * Pyrolytic graphite electrode
OECD category: Physical chemistry
Nontemplate DNA synthesis is a feature specific for a special type of DNA polymerase, terminal deoxynucleotidyl transferase (TdT). This enzyme is able. to randomly add nucleotides to a sintde-stranded DNA primer, provided the 3'OH end of the primer is accessible, Here we show that voltammetric analysis based on hanging mercury drop electrode (EPODE) and pyrolytic graphite electrode (PGE) enables to study this nontemplate DNA synthesis.
Permanent Link: http://hdl.handle.net/11104/0297794
Number of the records: 1