Electrochemical Study of Osmium Tetroxide Comptests Reactivity to DNA Bearing Butylacrylate

0506547 - BFÚ 2020 RIV CZ eng C - Conference Paper (international conference)
Havran, Luděk - Havranová-Vidláková, Pavlína - Špaček, Jan - Vítová, Lada - Hermanová, Monika - Fojta, Miroslav
Electrochemical Study of Osmium Tetroxide Comptests Reactivity to DNA Bearing Butylacrylate.
XXXVIII. Moderní elektrochemické metody. Sborník přednášek. Ústí nad Labem: Best Servis, 2018 - (Navrátil, T.; Fojta, M.; Schwarzová, K.), Č. 2018 (2018), s. 66-69. ISBN 978-80-905221-6-9.
[Moderní elektrochemické metody /38./. Jetřichovice (CZ), 21.05.2018-25.05.2018]
R&D Projects: GA ČR GA15-08434S; GA ČR GBP206/12/G151; GA MŠMT EF15_003/0000477
Institutional support: RVO:68081707
Keywords : Osmium tetroxide complexes * Chemically modified DNA * Voltammetry * Butyl acrylate
OECD category: Physical chemistry

Coniplexes of osmium tetroside with nitrogen ligands (Os,L e.g.. with 2,2'-bipyridine (bpy) find application in redox labelling of DNA.. probing of DNA structure, and in studies of DNA interaction with other molecules Os,L preferentially react with pyrimidines in single strand DNA producing electroactive adducts Primary reaction site for Os,L is C=C double bond in pyrimidine nucleobases. In this contribution we introduce a new two-step technique of DNA modification with Os,bpy, consisting in enzymatic construdion or DNA beating butyl acrylate (BA) moieties attached to uracil or T-deaza adenine, followed by chemical modification of a reactive C=C double bond in BA residue.
Permanent Link: http://hdl.handle.net/11104/0297779