Genome-wide C-SWAT library for high-throughput yeast genome tagging

0506546 - ÚFCH JH 2020 RIV GB eng J - Journal Article
Meurer, M. - Duan, Y. - Sass, E. - Kats, I. - Herbst, K. - Buchmuller, B.C. - Dederer, V. - Huber, F. - Kirrmaier, D. - Štefl, Martin - Van Laer, K. - Dick, T.P. - Lemberg, M. K. - Khmelinskii, A. - Levy, E.D. - Knop, M.
Genome-wide C-SWAT library for high-throughput yeast genome tagging.
Nature Methods. Roč. 15, č. 8 (2018), s. 598-600. ISSN 1548-7091. E-ISSN 1548-7105
Institutional support: RVO:61388955
Keywords : green fluorescent protein * saccharomyces-cerevisiae * gene deletion * global analysis * budding yeast * pcr * expression
OECD category: Physical chemistry
Impact factor: 28.467, year: 2018
Method of publishing: Limited access

Here we describe a C-SWAT library for high-throughput tagging of Saccharomyces cerevisiae open reading frames (ORFs). In 5,661 strains, we inserted an acceptor module after each ORF that can be efficiently replaced with tags or regulatory elements. We validated the library with targeted sequencing and tagged the proteome with bright fluorescent proteins to quantify the effect of heterologous transcription terminators on protein expression and to localize previously undetected proteins.
Permanent Link: http://hdl.handle.net/11104/0297778