The comprehensive in vitro evaluation of eight different calcium phosphates: Significant parameters for cell behavior

0506357 - ÚSMH 2020 RIV US eng J - Journal Article
Šupová, Monika - Suchý, Tomáš - Sucharda, Zbyněk - Filová, Elena - der Kinderen, J.N.L.M. - Steinerová, Marie - Bačáková, Lucie - Martynková, G.S.
The comprehensive in vitro evaluation of eight different calcium phosphates: Significant parameters for cell behavior.
Journal of the American Ceramic Society. Roč. 102, č. 5 (2019), s. 2882-2904. ISSN 0002-7820. E-ISSN 1551-2916
R&D Projects: GA MZd(CZ) NV15-25813A; GA MZd(CZ) NV15-32497A; GA ČR(CZ) GA15-01558S; GA MŠMT(CZ) ED1.1.00/02.0109
Grant - others:OPPK(XE) CZ.2.16/3.1.00/21538
Program: OPPK
Institutional support: RVO:67985891 ; RVO:67985823
Keywords : Calcium phosphate * Nanoparticles * Osteoblasts * In vitro tests
OECD category: Medical engineering; Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction) (FGU-C)
Impact factor: 3.502, year: 2019
Method of publishing: Limited access
https://ceramics.onlinelibrary.wiley.com/doi/abs/10.1111/jace.16110

Eight different calcium phosphate nanoparticles, namely bovine bone bioapatite calcined at 500, 600, and 700 degrees C, Mg-doped brushite, fluorinated calcium phosphate, Ca-deficient hydroxyapatite, hydroxyapatite, and tricalcium phosphate, were characterized employing physico-chemical methods. Their cytocompatibility was evaluated under human osteoblast-like cell line MG-63 culture conditions in elution media and via the direct interaction of cells with calcium phosphate nanoparticles. The main objective was to determine the correlation of the cell indices with the differently determined physical and chemical parameters of the calcium phosphates. Chemical composition, which contributes toward pH changes, and the calcium ion concentration in the medium appear to make up particularly significant factors, moreover, it was proved that the number of material types represents a further important aspect. In the case of a large number of material types, almost no correlation was determined between the analyzed parameters, however, in the case of a small number of apatite types, several positive correlations were found. It can be concluded that it is not possible to identify any monitored parameters that had a major impact on cell behavior or, at least, such an effect which can be generalized. It appears more likely that cell behavior is affected by the interplay of various parameters.
Permanent Link: http://hdl.handle.net/11104/0297624