Toxicity evaluation of monodisperse PEGylated magnetic nanoparticles for nanomedicine

0506160 - ÚMCH 2020 RIV GB eng J - Journal Article
Patsula, Vitalii - Tulinská, J. - Trachtová, Š. - Kuricová, M. - Lišková, A. - Španová, A. - Čiampor, F. - Vavra, I. - Rittich, B. - Ursinyova, M. - Dusinska, M. - Ilavská, S. - Horváthová, M. - Masanova, V. - Uhnakova, I. - Horák, Daniel
Toxicity evaluation of monodisperse PEGylated magnetic nanoparticles for nanomedicine.
Nanotoxicology. Roč. 13, č. 4 (2019), s. 510-526. ISSN 1743-5390. E-ISSN 1743-5404
R&D Projects: GA ČR(CZ) GA17-04918S; GA MŠMT(CZ) LQ1604; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:61389013
Keywords : iron oxide nanoparticles * PCR inhibition * cell interaction and uptake
OECD category: Polymer science
Impact factor: 4.925, year: 2019
Method of publishing: Limited access
https://www.tandfonline.com/doi/full/10.1080/17435390.2018.1555624

Innovative nanotechnology aims to develop particles that are small, monodisperse, smart, and do not cause unintentional side effects. Uniform magnetic Fe3O4 nanoparticles (12 nm in size) were prepared by thermal decomposition of iron(III) oleate. To make them colloidally stable and dispersible in water and cell culture medium, they were modified with phosphonic acid- (PA) and hydroxamic acid (HA)-terminated poly(ethylene glycol) yielding PA-PEG@Fe3O4 and HA-PEG@Fe3O4 nanoparticles. Conventional gama-Fe2O3 particles were prepared as a control. Advanced techniques were used to evaluate the properties and safety of the particles. Completeness of the nanoparticle coating was tested by real-time polymerase chain reaction. Interaction of the particles with primary human peripheral blood cells, cellular uptake, cytotoxicity, and immunotoxicity were also investigated. Amount of internalized iron in peripheral blood mononuclear cells was 72, 38, and 25 pg Fe/cell for HA-PEG@Fe3O4,gama-Fe2O3, and PA-PEG@Fe3O4, respectively. Nanoparticles were localized within the cytoplasm and in the extracellular space. No cytotoxic effect of both PEGylated nanoparticles was observed (0.12–75 μg/cm2) after 24 and 72-h incubation. Moreover, no suppressive effect was found on the proliferative activity of T-lymphocytes and T-dependent B-cell response, phagocytic activity of monocytes and granulocytes, and respiratory burst of phagocytes. Similarly, no cytotoxic effect of gama-Fe2O3 particles was observed. However, they suppressed the proliferative activity of T-lymphocytes (75 μg/cm2, 72 h) and also decreased the phagocytic activity of monocytes (15 μg/cm2 24 h, 3–75 μg/cm2 72 h). We thus show that newly developed particles have great potential especially in cancer diagnostics and therapy.
Permanent Link: http://hdl.handle.net/11104/0297622