Antimicrobial peptides prevent bacterial biofilm formation on the surface of polymethylmethacrylate bone cement

0505762 - ÚOCHB 2020 RIV GB eng J - Journal Article
Volejníková, Andrea - Melicherčík, P. - Nešuta, Ondřej - Vaňková, Eva - Bednárová, Lucie - Rybáček, Jiří - Čeřovský, Václav
Antimicrobial peptides prevent bacterial biofilm formation on the surface of polymethylmethacrylate bone cement.
Journal of Medical Microbiology. Roč. 68, č. 6 (2019), s. 961-972. ISSN 0022-2615. E-ISSN 1473-5644
R&D Projects: GA MZd(CZ) NV16-27726A
Institutional support: RVO:61388963
Keywords : antimicrobial peptides * antibiotics * polymethylmethacrylate bone cement * biofilm * implant-related infections
OECD category: Microbiology
Impact factor: 2.156, year: 2019
Method of publishing: Limited access
https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.001000

Purpose: Antibiotic-loaded polymethylmethacrylate-based bone cement has been implemented in orthopaedics to cope with implant-related infections associated with the formation of bacterial biofilms. In the context of emerging bacterial resistance to current antibiotics, we examined the efficacy of short antimicrobial peptide-loaded bone cement in inhibiting bacterial adhesion and consequent biofilm formation on its surface. Methodology: The ability of alpha-helical antimicrobial peptides composed of 12 amino acid residues to prevent bacterial biofilm [methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis, Pseudomonas aeruginosa and Escherichia coli] formation on the surface of model implants made from polymethylmethacrylate-based bone cement was evaluated by colony-forming unit (c.f) counting of bacteria released by sonication from the biofilms formed on their surfaces. The biofilms on model implant surfaces were also visualized by light microscopy after staining with tetrazolium dye (MTT) and by scanning electron microscopy. Results: When incorporated in the implants, these peptides caused a mean reduction in the number of bacterial cells attached to implants' surfaces (by five orders of magnitude), and 88 % of these implants showed no bacterial adhesion after being exposed to growth media containing various bacteria. Conclusion: The results showed that the antibiofilm activity of these peptides was comparable to that of the antibiotics, but the peptides exhibited broader specificity than the antibiotics. Given the rapid development of antibiotic resistance, antimicrobial peptides show promise as a substitute for antibiotics for loading into bone cements.
Permanent Link: http://hdl.handle.net/11104/0297152