Pinstatic Acid Promotes Auxin Transport by Inhibiting PIN Internalization

0505651 - ÚEB 2020 RIV US eng J - Journal Article
Oochi, A. - Hajný, Jakub - Fukui, K. - Nakao, Y. - Gallei, M. - Quareshy, M. - Takahashi, K. - Kinoshita, T. - Harborough, S. R. - Kepinski, S. - Kasahara, H. - Napier, R. - Friml, J. - Hayashi, K.
Pinstatic Acid Promotes Auxin Transport by Inhibiting PIN Internalization.
Plant Physiology. Roč. 180, č. 2 (2019), s. 1152-1165. ISSN 0032-0889. E-ISSN 1532-2548
Institutional support: RVO:61389030
Keywords : intracellular trafficking * mediates endocytosis * arabidopsis * proteins * efflux * biosynthesis * responses * localization * complex
OECD category: Biochemistry and molecular biology
Impact factor: 6.902, year: 2019
Method of publishing: Open access
http://dx.doi.org/10.1104/pp.19.00201

Polar auxin transport plays a pivotal role in plant growth and development. PIN-FORMED (PIN) auxin efflux carriers regulate directional auxin movement by establishing local auxin maxima, minima, and gradients that drive multiple developmental processes and responses to environmental signals. Auxin has been proposed to modulate its own transport by regulating subcellular PIN trafficking via processes such as clathrin-mediated PIN endocytosis and constitutive recycling. Here, we further investigated the mechanisms by which auxin affects PIN trafficking by screening auxin analogs and identified pinstatic acid (PISA) as a positive modulator of polar auxin transport in Arabidopsis (Arabidopsis thaliana). PISA had an auxin-like effect on hypocotyl elongation and adventitious root formation via positive regulation of auxin transport. PISA did not activate SCFTIR1/AFB signaling and yet induced PIN accumulation at the cell surface by inhibiting PIN internalization from the plasma membrane. This work demonstrates PISA to be a promising chemical tool to dissect the regulatory mechanisms behind subcellular PIN trafficking and auxin transport.
Permanent Link: http://hdl.handle.net/11104/0297085