Mouse ANKRD31 Regulates Spatiotemporal Patterning of Meiotic Recombination Initiation and Ensures Recombination between X and Y Sex Chromosomes

0505620 - ÚMG 2020 RIV US eng J - Journal Article
Papanikos, F. - Clement, J. A. J. - Testa, E. - Ravindranathan, R. - Grey, C. - Dereli, I. - Bondarieva, A. - Valerio-Cabrera, S. - Stanzione, M. - Schleiffer, A. - Jansa, Petr - Lustyk, Diana - Fei, J. - Adams, I. R. - Forejt, Jiří - Barchi, M. - de Massy, B. - Toth, A.
Mouse ANKRD31 Regulates Spatiotemporal Patterning of Meiotic Recombination Initiation and Ensures Recombination between X and Y Sex Chromosomes.
Molecular Cell. Roč. 74, č. 5 (2019), s. 1069-1085. ISSN 1097-2765. E-ISSN 1097-4164
R&D Projects: GA ČR GA13-08078S
Institutional support: RVO:68378050
Keywords : strand break formation * pseudoautosomal region * read alignment * elements * gene * conservation * surveillance * expression * responses * features
OECD category: Biochemistry and molecular biology
Impact factor: 15.584, year: 2019
Method of publishing: Limited access

Orderly segregation of chromosomes during meiosis requires that crossovers form between homologous chromosomes by recombination. Programmed DNA double-strand breaks (DSBs) initiate meiotic recombination. We identify ANKRD31 as a key component of complexes of DSB-promoting proteins that assemble on meiotic chromosome axes. Genome-wide, ANKRD31 deficiency causes delayed recombination initiation. In addition, loss of ANKRD31 alters DSB distribution because of reduced selectivity for sites that normally attract DSBs. Strikingly, ANKRD31 deficiency also abolishes uniquely high rates of recombination that normally characterize pseudoautosomal regions (PARs) of X and Y chromosomes. Consequently, sex chromosomes do not form cross-overs, leading to chromosome segregation failure in ANKRD31-deficient spermatocytes. These defects co-occur with a genome-wide delay in assembling DSB-promoting proteins on autosome axes and loss of a specialized PAR-axis domain that is highly enriched for DSB-promoting proteins in wild type. Thus, we propose a model for spatiotemporal patterning of recombination by ANKRD31-dependent control of axis-associated DSB-promoting proteins.
Permanent Link: http://hdl.handle.net/11104/0297077