Method of identification of European freshwater fish and hybrides in biological materials by S7ICAPS method

0505460 - ÚBO 2020 RIV eng P - Patent Document
Mendel, Jan - Halačka, Karel - Vetešník, Lukáš
Method of identification of European freshwater fish and hybrides in biological materials by S7ICAPS method.
2019. Owner: Ústav biologie obratlovců AV ČR, v. v. i. Date of the patent acceptance: 08.05.2019. Patent Number: EP 3199643. Territorial Protection: evropský patent podle Evropské patentové úmluvy .
R&D Projects: GA TA ČR TG03010048; GA ČR GP206/09/P608
Grant - others:GA AV ČR(CZ) M200930901
Keywords : nDNA determination * freshwater fish * S7iCAPS method
OECD category: Zoology
https://worldwide.espacenet.com/publicationDetails/originalDocument?FT=D&date=20190508&DB=&locale=en_EP&CC=EP&NR=3199643B1&KC=B1&ND=5

A method of identification of European freshwater fish species/lineages in biological materials and fish products using a method based on amplification of intron 1 of the gene encoding the S7 ribosomal protein RPS7 with primers which bind internally in the intron and in which the amplicons can be distinguished by length or by a specific restriction pattern of partial genomic sequence of the first intron of the S7 gene. The method consists in using of genus or group-specific primer sets, unique combination design of variable InDel-CAPS and/or SNP-CAPS markers and is qualitative.
Patent know-how was utilized in the Contract on application of certified methodology No. R15/2017 and the Contract on application of verified technology No. R22/2018 which were concluded between the Institute of Vertebrate Biology, Czech Academy of Sciences and the company BioFish s.r.o. (Ltd.), based at Horní Paseka 40, 548 01 Ledeč nad Sázavou, Czech Republic VAT: CZ60930527, on September 6th, 2017 and December 19th, 2018, respectively. It is also used within the DNA service which is provided by the Institute of Vertebrate Biology to professional and lay public in CR and abroad. The service of fish identification according to the invention was provided to subjects as for example the University of Presov 17. Novembra 1, 081 16 Prešov, Slovakia, VAT: SK2020980082 (contract of November 2nd, 2017); Society for Nature Conservation and Landscape Ecology (GNL), Dorfstrasse 31, D-17237 Kratzeburg, Germany VAT: DE165399174 (contract of September 21st, 2018); the University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters of the University of South Bohemia, Branišovská 1645/31a, 370 05 České Budějovice, Czech Republic, VAT: CZ60076658 (contract of February 27th, 2019); Ostrava Zoological Garden and Botanical Park, Michálkovická 2081/197, 710 00 Ostrava, VAT: CZ00373249 (contract of May 25th, 2020); Podyjí National Park, Na Vyhlídce 5, 669 01 Znojmo, VAT: CZ00837971 (contract of June 4th, 2020); Zdeněk Suchý, Družstevní 37, Olomouc, zdesu@atlas.cz (contract of July 27th, 2020); Biology Centre CAS, Institute of Hydrobiology, Na Sádkách 7, 370 05 České Budějovice, VAT: CZ60077344 (contracts of August 6th and November 3rd, 2020); Czech Fishing Union, Jahnova 14, 709 00 Ostrava, VAT: CZ00434167 (contracts of August, 2021 and 2022); Nature Conservation Agency of the Czech Republic, Kaplanova 1931/1, 148 00 Praha, CZ00434167 (PPK-1033a/25/23 contract of May, 2023); FROV, Zátiší 728/II, 389 25 Vodňany, VAT: CZ60076658 (contracts of August and October 2023); etc.

Permanent Link: http://hdl.handle.net/11104/0296956