A multifunctional graphene oxide platform for targeting cancer

0505100 - ÚMCH 2020 RIV CH eng J - Journal Article
Bugárová, N. - Špitálsky, Z. - Mičušík, M. - Bodík, M. - Šiffalovič, P. - Koneracká, M. - Závišová, V. - Kubovčíková, M. - Kajanová, I. - Zaťovičová, M. - Pastoreková, S. - Šlouf, Miroslav - Majková, E. - Omastová, M.
A multifunctional graphene oxide platform for targeting cancer.
Cancers (Basel). Roč. 11, č. 6 (2019), s. 1-19, č. článku 753. E-ISSN 2072-6694
R&D Projects: GA TA ČR(CZ) TE01020118; GA MŠMT(CZ) LO1507
Institutional support: RVO:61389013
Keywords : graphene oxide * magnetic nanoparticles * monoclonal antibodies
OECD category: Polymer science
Impact factor: 6.126, year: 2019
Method of publishing: Open access
https://www.mdpi.com/2072-6694/11/6/753/pdf

Diagnosis of oncological diseases remains at the forefront of current medical research. Carbonic Anhydrase IX (CA IX) is a cell surface hypoxia-inducible enzyme functionally involved in adaptation to acidosis that is expressed in aggressive tumors. Hence, it can be used as a tumor biomarker. Herein, we propose a nanoscale graphene oxide (GO) platform functionalized with magnetic nanoparticles and a monoclonal antibody specific to the CA IX marker. The GO platforms were prepared by a modified Hummers and Offeman method from exfoliated graphite after several centrifugation and ultrasonication cycles. The magnetic nanoparticles were prepared by a chemical precipitation method and subsequently modified. Basic characterization of GO, such as the degree of oxidation, nanoparticle size and exfoliation, were determined by physical and chemical analysis, including X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), energy dispersive X-ray analysis (EDX), and atomic force microscopy (AFM). In addition, the size and properties of the poly-L-lysine-modified magnetic nanoparticles were characterized. The antibody specific to CA IX was linked via an amidic bond to the poly-L-lysine modified magnetic nanoparticles, which were conjugated to GO platform again via an amidic bond. The prepared GO-based platform with magnetic nanoparticles combined with a biosensing antibody element was used for a hypoxic cancer cell targeting study based on immunofluorescence.
Permanent Link: http://hdl.handle.net/11104/0298185