Tubes for detection of cholinesterase inhibitors - unique effects of Neusilin on the stability of butyrylcholinesterase-impregnated carriers

0504873 - ÚMCH 2020 RIV US eng J - Journal Article
Zeman, J. - Vetchý, D. - Pavloková, S. - Franc, A. - Pitschmann, V. - Dominik, M. - Urbanová, Martina - Šeděnková, Ivana
Tubes for detection of cholinesterase inhibitors - unique effects of Neusilin on the stability of butyrylcholinesterase-impregnated carriers.
Enzyme and Microbial Technology. Roč. 128, September (2019), s. 26-33. ISSN 0141-0229. E-ISSN 1879-0909
Institutional support: RVO:61389013
Keywords : cholinesterase * detection * enzyme
OECD category: Polymer science
Impact factor: 3.448, year: 2019
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0141022919300699?via%3Dihub

Detection tubes are small devices for the colorimetric enzymatic detection of cholinesterase inhibitors such as sarin, soman, VX nerve agents and substances denoted as Novichok. These detectors contain carriers in the form of pellets with immobilized cholinesterase, substrate and detection reagent. Their advantages are portability, sensitivity and simplicity, enabling fast detection of such compounds from air and water in case of a terrorist attack or war. In general, maintaining the stability of an enzyme for a longer time is very problematic. Therefore, its further enhancement is required for safety and financial reasons. In this study, the stability of our patented carriers in the form of pellets with immobilized butyrylcholinesterase containing an increasing amount of the unique sorbent Neusilin® US2 was evaluated. The samples containing Neusilin maintained the stability of the immobilized enzyme for a longer time even at higher temperature and humidity than the currently commercially used carrier without Neusilin, allowing improved detection of nerve agents.
Permanent Link: http://hdl.handle.net/11104/0296617