0504656 - FZÚ 2020 RIV NL eng J - Článek v odborném periodiku
Procházka, Václav - Matějka, Roman - Ižák, Tibor - Szabó, Ondrej - Štěpanovská, Jana - Filová, Elena - Bačáková, Lucie - Jirásek, Vít - Kromka, Alexander
Nanocrystalline diamond-based impedance sensors for real-time monitoring of adipose tissue-derived stem cells.
Colloids and Surfaces B-Biointerfaces. Roč. 177, May (2019), s. 130-136. ISSN 0927-7765. E-ISSN 1873-4367
Grant CEP: GA MZd(CZ) NV15-33018A; GA MŠMT EF16_019/0000760
Grant ostatní: OP VVV - SOLID21(XE) CZ.02.1.01/0.0/0.0/16_019/0000760
Institucionální podpora: RVO:68378271 ; RVO:67985823
Klíčová slova: diamond film * biosensor * stem cells * impedance spectroscopy
Obor OECD: Electrical and electronic engineering; Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials (FGU-C)
Impakt faktor: 4.389, rok: 2019 ; AIS: 0.681, rok: 2019
Způsob publikování: Omezený přístup
Web výsledku:
https://doi.org/10.1016/j.colsurfb.2019.01.048DOI: https://doi.org/10.1016/j.colsurfb.2019.01.048

Here we present a diamond-based impedance sensor with built-in gold IDT as a promising platform for simultaneous electrical and optical monitoring of adipose tissue-derived stem cells (ASCs). The impedance spectra were collected in a wide frequency from 100 Hz to 50 kHz for 90 h of cell cultivation. Absolute impedance spectra were analyzed in terms of cell properties monitored by a high-resolution digital camera. The sensors detected three phases of cell growth: the phase of cell attachment and spreading, the phase of cell proliferation, and the stationary phase without significant changes in cell number. These results were confirmed by simultaneous live cell imaging. The design of the sensing electrode is discussed, pointing out its enhanced sensitivity for a certain case. The diamond-based sensor appeared to be more sensitive for detecting the cell-substrate interaction in the first phase of cell growth, while the control system was more sensitive in the second phase of cell growth.
Trvalý link: http://hdl.handle.net/11104/0296238