Optical imaging and localization of prospective scattering labels smaller than a single protein

0504562 - ÚFE 2020 RIV GB eng J - Journal Article
Holanová, Kristýna - Vala, Milan - Piliarik, Marek
Optical imaging and localization of prospective scattering labels smaller than a single protein.
Optics and Laser Technology. -, č. 109 (2019), s. 323-327. ISSN 0030-3992. E-ISSN 1879-2545
R&D Projects: GA MŠMT(CZ) LL1602
Institutional support: RVO:67985882
Keywords : iSCAT * Nanoparticle detection * Interference scattering microscopy
OECD category: Optics (including laser optics and quantum optics)
Impact factor: 3.233, year: 2019
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S0030399218310818?via%3Dihub

Labeling of biological matter is a key technique enabling visualization and real-time tracking of dynamic processes beyond the diffraction limit. While the precision of fluorescence-based localization microscopy is limited by signal saturation, a new generation of small scattering labels (such as gold nanoparticles) have brought the localization precision below the size of a single protein. However, the size of scattering labels is also the most obvious handicap, as they are usually significantly larger than the resulting localization precision. We address this inconsistency and demonstrate detection and precise localization of gold nanoparticles of only 2 nm in diameter using interferometric detection of scattering (iSCAT). This size is considerably smaller than the size of a small protein and in combination with the possibility of tracking the nanoparticle position, we foresee a new concept of detailed studies of biomolecular systems in the future
Permanent Link: http://hdl.handle.net/11104/0296168