0504554 - ÚFCH JH 2020 RIV CH eng J - Journal Article
Jirka, Ivan - Kopová, Ivana - Kubát, Pavel - Tabor, Edyta - Bačáková, Lucie - Bouša, Milan - Sajdl, P.
The Photodynamic Properties and the Genotoxicity of Heat-Treated Silicalite-1 Films.
Materials. Roč. 12, č. 4 (2019), č. článku 567. ISSN 1996-1944. E-ISSN 1996-1944
R&D Projects: GA ČR(CZ) GA16-02681S; GA MŠMT(CZ) EF16_013/0001821
Institutional support: RVO:61388955 ; RVO:67985823
Keywords : induced oxidative stress * singlet oxygen * stainless-steel * dna-damage * propene oligomerization
OECD category: Physical chemistry; Biomaterials (as related to medical implants, devices, sensors) (FGU-C)
Impact factor: 3.057, year: 2019 ; AIS: 0.543, rok: 2019
Method of publishing: Open access
DOI: https://doi.org/10.3390/ma12040567

We investigated the use of a supported silicalite-1 film (SF) as a promising coating for metallic materials used in the fabrication of prostheses. The role of carbonaceous residua present on high-temperature calcined-SF in generating singlet oxygen for future use as a sterilization method has also been addressed, and the potential genotoxicity of these residua in osteoblast-like cells has been investigated. Calcination of as-synthesized SF induced the appearance of a rather complicated mixture of aliphatic and aromatic species on its outer surface. A series of variously volatile polycyclic aromatic hydrocarbons (PAH), including naphthalene, fluorene, phenanthrene, anthracene, fluoranthene, and pyrene, were identified in micromole concentrations. Irradiation of these PAHs on calcined-SF immersed in air-saturated chloroform led to the formation of very low concentrations of singlet oxygen. However, an increased level of DNA damage was observed on calcined-SF by immunofluorescence staining of phosphorylated histone H2AX analyzed by flow cytometry.
Permanent Link: http://hdl.handle.net/11104/0296157