Substitution-Inert Polynuclear Platinum Complexes Act as Potent Inducers of Condensation/Aggregation of Short Single- and Double-Stranded DNA and RNA Oligonucleotides

0504519 - BFÚ 2020 RIV DE eng J - Journal Article
Malina, Jaroslav - Farrell, N.P. - Brabec, Viktor
Substitution-Inert Polynuclear Platinum Complexes Act as Potent Inducers of Condensation/Aggregation of Short Single- and Double-Stranded DNA and RNA Oligonucleotides.
Chemistry - A European Journal. Roč. 25, č. 12 (2019), s. 2995-2999. ISSN 0947-6539. E-ISSN 1521-3765
R&D Projects: GA ČR GA16-03517S
Institutional support: RVO:68081707
Keywords : phosphate clamp * recognition * selectivity * length
OECD category: Physical chemistry
Impact factor: 4.857, year: 2019
Method of publishing: Limited access
https://onlinelibrary.wiley.com/doi/abs/10.1002/chem.201806276

Compounds condensing DNA and RNA molecules can essentially affect important biological processes including DNA replication and transcription. Here, this work shows with the aid of total intensity light scattering, gel electrophoresis, and atomic force microscopy (AFM) that the substitution-inert polynuclear platinum complexes (SI-PPCs), particularly [{trans-Pt(NH3)(2)(NH2(CH2)(6)- NH3+)}(2)--{trans-Pt(NH3)(2)(NH2(CH2)(6)NH2)(2)}](8+) (Triplatin NC), exhibit an unprecedented high potency to condense/aggregate fragments of DNA and RNA as short as 20 base pairs. SI-PPCs condensates are distinctive from those generated by the naturally occurring polyamines (commonly used DNA compacting/condensing agents). Collectively, the results further confirm that SI-PPCs are very efficient inducers of condensation of DNA and RNA, including their short fragments that might have potential in gene therapy, biotechnology, and bionanotechnology. Moreover, the data confirm the structural advantages of the phosphate clamp, with a well-defined rigid DNA recognition motif in initiating condensation and aggregation phenomena on oligonucleotides.
Permanent Link: http://hdl.handle.net/11104/0296135