Quantitative insights into the cyanobacterial cell economy

0504331 - ÚVGZ 2020 RIV GB eng J - Journal Article
Zavřel, Tomáš - Faizi, M. - Loureiro, C. - Poschmann, G. - Stuehler, K. - Sinětova, Maria A. - Zorina, A. - Steuer, R. - Červený, Jan
Quantitative insights into the cyanobacterial cell economy.
eLife. Roč. 8, feb (2019), č. článku e42508. ISSN 2050-084X. E-ISSN 2050-084X
R&D Projects: GA ČR(CZ) GA18-24397S
Research Infrastructure: CzeCOS II - 90061
Institutional support: RVO:86652079
Keywords : sp pcc 6803 * bacterial-growth laws * gene-expression * resource-allocation * chlorophyll fluorescence * trade-offs * light * cultivation * model * photobioreactor
OECD category: Microbiology
Impact factor: 7.080, year: 2019
Method of publishing: Open access
https://elifesciences.org/articles/42508

Phototrophic microorganisms are promising resources for green biotechnology. Compared to heterotrophic microorganisms, however, the cellular economy of phototrophic growth is still insufficiently understood. We provide a quantitative analysis of light-limited, light-saturated, and light-inhibited growth of the cyanobacterium Synechocystis sp. PCC 6803 using a reproducible cultivation setup. We report key physiological parameters, including growth rate, cell size, and photosynthetic activity over a wide range of light intensities. Intracellular proteins were quantified to monitor proteome allocation as a function of growth rate. Among other physiological acclimations, we identify an upregulation of the translational machinery and downregulation of light harvesting components with increasing light intensity and growth rate. The resulting growth laws are discussed in the context of a coarse-grained model of phototrophic growth and available data obtained by a comprehensive literature search. Our insights into quantitative aspects of cyanobacterial acclimations to different growth rates have implications to understand and optimize photosynthetic productivity.
Permanent Link: http://hdl.handle.net/11104/0295991