Mitochondrial Nucleoids: Superresolution microscopy analysis

0504242 - FGÚ 2020 RIV GB eng J - Journal Article
Ježek, Petr - Špaček, Tomáš - Tauber, Jan - Pavluch, Vojtěch
Mitochondrial Nucleoids: Superresolution microscopy analysis.
International Journal of Biochemistry and Cell Biology. Roč. 106, Jan (2019), s. 21-25. ISSN 1357-2725. E-ISSN 1878-5875
R&D Projects: GA ČR(CZ) GA16-04788S; GA ČR(CZ) GA17-01813S
Institutional support: RVO:67985823
Keywords : mtDNA * nucleoids * 3D superresolution microscopy * TFAM
OECD category: Cell biology
Impact factor: 3.673, year: 2019
Method of publishing: Limited access
https://doi.org/10.1016/j.biocel.2018.10.012

The mitochondrion owns an autonomous genome. Double-stranded circular mitochondrial DNA (mtDNA) is organized in complexes with a packing/stabilizing transcription factor TFAM, having multiple roles, and proteins of gene expression machinery in structures called nucleoids. From hundreds to thousands nucleoids exist distributed in the matrix of mitochondria] reticulum network. A single mtDNA molecule contained within the single nucleoid is a currently preferred but questioned model. Nevertheless, mtDNA replication should lead transiently to its doubling within a nucleoid. However, nucleoid division has not yet been documented in detail. A 3D superresolution microscopy is required to resolve nucleoid biology occurring in similar to 100 nm space, having an advantage over electron microscopy tomography in resolving the particular protein components. We discuss stochastic vs. stimulated emission depletion microscopy yielding wide vs. narrow nucleoid size distribution, respectively. Nucleoid clustering into spheroids fragmented from the continuous mitochondria] network, likewise possible nucleoid attachment to the inner membrane is reviewed.
Permanent Link: http://hdl.handle.net/11104/0295920