Liraglutide and a lipidized analog of prolactin-releasing peptide show neuroprotective effects in a mouse model of beta-amyloid pathology

0504222 - FGÚ 2020 RIV NL eng J - Journal Article
Holubová, M. - Hrubá, L. - Popelová, A. - Bencze, Michal - Pražienková, V. - Gengler, S. - Kratochvílová, H. - Haluzík, M. - Železná, B. - Kuneš, Jaroslav - Hölscher, C. - Maletínská, L.
Liraglutide and a lipidized analog of prolactin-releasing peptide show neuroprotective effects in a mouse model of beta-amyloid pathology.
Neuropharmacology. Roč. 144, Jan (2019), s. 377-387. ISSN 0028-3908. E-ISSN 1873-7064
Institutional support: RVO:67985823
Keywords : Alzheimer's disease * Palm(11)-PrRP31 * APP/PS1 mice * beta-amyloid plaques * neuroinflammation * tau phosphorylation
OECD category: Neurosciences (including psychophysiology
Impact factor: 4.431, year: 2019
Method of publishing: Limited access
https://doi.org/10.1016/j.neuropharm.2018.11.002

Obesity and type 2 diabetes mellitus (T2DM) are important risk factors for Alzheimer's disease (AD). Drugs originally developed for T2DM treatment, e.g., analog of glucagon-like peptide 1 liraglutide, have shown neuroprotective effects in mouse models of AD. We previously examined the neuroprotective properties of palm(11)-PrRP31, an anorexigenic and glucose-lowering analog of prolactin-releasing peptide, in a mouse model of AD-like Tau pathology, THY-Tau22 mice. Here, we demonstrate the neuroprotective effects of palm(11)-PrRP31 in double transgenic APP/PS1 mice, a model of AD-like beta-amyloid (A beta) pathology. The 7-8-month-old APP/PS1 male mice were subcutaneously injected with liraglutide or palm(11)-PrRP31 for 2 months. Both the liraglutide and palm(11)-PrRP31 treatments reduced the A beta plaque load in the hippo campus. Palm(11)-PrRP31 also significantly reduced hippocampal microgliosis, consistent with our observations of a reduced A beta plaque load, and reduced cortical astrocytosis, similar to the treatment with liraglutide. Palm(11)-PrRP31 also tended to increase neurogenesis, as indicated by the number of doublecortin-positive cells in the hippocampus. After the treatment with both anorexigenic compounds, we observed a significant decrease in Tau phosphorylation at Thr231, one of the first epitopes phosphorylated in AD. This effect was probably caused by elevated activity of protein phosphatase 2A subunit C, the main Tau phosphatase. Both liraglutide and palm(11)-PrRP31 reduced the levels of caspase 3, which has multiple roles in the pathogenesis of AD. Palm(11)-PrRP31 increased protein levels of the pre-synaptic marker synaptophysin, suggesting that palm(11)-PrRP31 might help preserve synapses. These results indicate that palm(11)-PrRP31 has promising potential for the treatment of neurodegenerative diseases.
Permanent Link: http://hdl.handle.net/11104/0295906