Selective auxin agonists induce specific AUX/IAA protein degradation to modulate plant development

0504116 - ÚEB 2020 RIV US eng J - Journal Article
Vain, T. - Raggi, S. - Ferro, M. - Barange, D. K. - Kieffer, M. - Ma, Q. - Doyle, S. M. - Thelander, M. - Pařízková, Barbora - Novák, Ondřej - Ismail, A. A. - Enquist, P. A. - Rigal, A. - Łangowska, M. - Harborough, S. R. - Zhang, Y. - Ljung, K. - Callis, J. - Almqvist, F. - Kepinski, S. - Estelle, M. - Pauwels, L. - Robert, S.
Selective auxin agonists induce specific AUX/IAA protein degradation to modulate plant development.
Proceedings of the National Academy of Sciences of the United States of America. Roč. 116, č. 13 (2019), s. 6463-6472. ISSN 0027-8424. E-ISSN 1091-6490
R&D Projects: GA MŠMT(CZ) EF16_019/0000827
Institutional support: RVO:61389030
Keywords : Auxin * Chemical biology * Hormone perception * Prohormone * Selective agonist
OECD category: Biochemistry and molecular biology
Impact factor: 9.412, year: 2019
Method of publishing: Open access
http://doi.org/10.1073/pnas.1809037116

Auxin phytohormones control most aspects of plant development through a complex and interconnected signaling network. In the presence of auxin, AUXIN/INDOLE-3-ACETIC ACID (AUX/IAA) transcriptional repressors are targeted for degradation by the SKP1-CULLIN1-F-BOX (SCF) ubiquitin-protein ligases containing TRANSPORT INHIBITOR RESISTANT 1/AUXIN SIGNALING F-BOX (TIR1/AFB). CULLIN1-neddylation is required for SCF TIR1/AFB functionality, as exemplified by mutants deficient in the NEDD8-activating enzyme subunit AUXIN-RESISTANT 1 (AXR1). Here, we report a chemical biology screen that identifies small molecules requiring AXR1 to modulate plant development. We selected four molecules of interest, RubNeddin 1 to 4 (RN1 to4), among which RN3 and RN4 trigger selective auxin responses at transcriptional, biochemical, and morphological levels. This selective activity is explained by their ability to consistently promote the interaction between TIR1 and a specific subset of AUX/IAA proteins, stimulating the degradation of particular AUX/IAA combinations. Finally, we performed a genetic screen using RN4, the RN with the greatest potential for dissecting auxin perception, which revealed that the chromatin remodeling ATPase BRAHMA is implicated in auxin-mediated apical hook development. These results demonstrate the power of selective auxin agonists to dissect auxin perception for plant developmental functions, as well as offering opportunities to discover new molecular players involved in auxin responses.
Permanent Link: http://hdl.handle.net/11104/0295817