The role of chloroplasts in the oxidative stress that is induced by zearalenone in wheat plants - The functions of 24-epibrassinolide and selenium in the protective mechanisms

0503951 - ÚEB 2020 RIV FR eng J - Journal Article
Filek, M. - Sieprawska, A. - Kościelniak, J. - Oklešťková, Jana - Jurczyk, B. - Telk, A. - Biesaga-Kościelniak, J. - Janeczko, A.
The role of chloroplasts in the oxidative stress that is induced by zearalenone in wheat plants - The functions of 24-epibrassinolide and selenium in the protective mechanisms.
Plant Physiology and Biochemistry. Roč. 137, APR (2019), s. 84-92. ISSN 0981-9428
Institutional support: RVO:61389030
Keywords : Brassinosteroids * Chloroplasts * Hsp90 * Photosynthesis * Selenium * Wheat * Zearalenone
OECD category: Plant sciences, botany
Impact factor: 3.720, year: 2019
Method of publishing: Open access
http://doi.org/10.1016/j.plaphy.2019.01.030

This study focused on the idea that the toxic effect of zearalenone (ZEA) and the protective actions of the brassinosteroid 24-epibrassinolide (EBR) as well as selenium are dependent on its accumulation in chloroplasts to a high degree. These organelles were isolated from the leaves of oxidative stress-sensitive and stress-tolerant wheat cultivars that had been grown from grains that had been incubated in a solution of ZEA (30 μM), Na 2 SeO 4 (Se, 10 μM), EBR (0.1 μM) or in a mixture of ZEA with Se or EBR. Ultra-high performance liquid chromatography techniques indicated that ZEA was adsorbed in higher amounts in the chloroplasts in the sensitive rather than tolerant cultivar. Although the brassinosteroids and Se were also accumulated in the chloroplasts, higher levels were only found in the tolerant cultivar. The application of EBR increased the homocastasterone content, especially in the chloroplasts of the tolerant plant and after the addition of ZEA. The presence of both protectants caused a decrease in the ZEA content in studied organelles and resulted in diminishing of the oxidative stress (i.e. changes in the activity of the antioxidative enzymes). Moreover, a recovery of photosystem II and decrease in the negative impact of ZEN on Hsp90 transcript accumulation was observed in plants.
Permanent Link: http://hdl.handle.net/11104/0295761