Kocuria Bacterial Isolates from Radioactive Springs of Jachymov spa (Joachimsthal) as Sources of Polyunsaturated Fatty Acids

0503944 - MBÚ 2020 RIV US eng J - Journal Article
Řezanka, Tomáš - Gharwalová, L. - Nováková, G. - Kolouchová, I. - Uhlík, O. - Sigler, Karel
Kocuria Bacterial Isolates from Radioactive Springs of Jachymov spa (Joachimsthal) as Sources of Polyunsaturated Fatty Acids.
Lipids. Roč. 54, 2-3 (2019), s. 177-187. ISSN 0024-4201. E-ISSN 1558-9307
R&D Projects: GA ČR(CZ) GA17-00027S
Institutional support: RVO:61388971
Keywords : Arachidonic and eicosapentaenoic acids * Kocuria sp * Polyunsaturated fatty acids
OECD category: Microbiology
Impact factor: 1.919, year: 2019
Method of publishing: Limited access
https://aocs.onlinelibrary.wiley.com/doi/abs/10.1002/lipd.12136

Four bacterial isolates, which produced polyunsaturated fatty acids (PUFA), were isolated from water samples of radioactive springs collected from Jachymov spa. Jachymov (Sankt Joachimsthal) is a city in northwestern Bohemia, where Marie and Pierre Curie isolated radium in 1898 from the mineral uraninite. To date, four springs (Agricola, Behounek, C1, and Curie) have been used for spa purposes, that is for the treatment of nervous and rheumatic disorders by constantly produced radioactive gas radon (Rn-222) dissolved in the water. The radioactivity reaches 24 kBq/L. Using 16S rRNA gene sequence analysis, all four isolates were identified as members of the genus Kocuria, with two isolates designated 208 and 401 affiliated with Kocuria kristinae, while isolates 101 and 301 most likely with K. rhizophila. The content of fatty acids in polar lipids was determined by gas chromatography-mass spectrometry (GC-MS) and two PUFA, that is arachidonic and eicosapentaenoic, were identified. The position of double bonds was confirmed by GC-MS of 3-pyridylcarbinol (formerly picolinyl) esters. We assume that all four isolates of Kocuria produce PUFA to increase the stability of cell membranes, which may be impaired by the reaction of the reactive oxygen species. These can arise, for example, because of radiation during Rn-222 decay.
Permanent Link: http://hdl.handle.net/11104/0295702