Combination of cobalt bis(dicarbollide) amino derivative and antibiotic effect on growth of staphylococcus epidermidis

0503776 - ÚACH 2020 RIV CZ eng C - Conference Paper (international conference)
Lokočová, K. - Vaňková, E. - Šícha, Václav - Masák, J.
Combination of cobalt bis(dicarbollide) amino derivative and antibiotic effect on growth of staphylococcus epidermidis.
International Conference on Chemical Technology. In: Proceedings of the 6th International Conference on Chemical Technology. Prague: Czech Society of Industrial Chemistry, 2018 - (Veselý, M.; Hrdlička, Z.; Hanika, J.; Lubojacký, J.), s. 91-94. ISBN 978-80-86238-77-7. ISSN 2336-8128.
[International Conference on Chemical Technology /6./. Mikulov (CZ), 16.04.2018-18.04.2018]
Institutional support: RVO:61388980
Keywords : cobalt bis(dicarbollide) * staphylococcus genus
OECD category: Inorganic and nuclear chemistry
https://www.icct.cz/AngiologyKlon-ICCT/media/system/2018/ICCT-2018-Proceedings.pdf

Nosocomial infections are often caused by bacteria from the Staphylococcus genus. The clinically relevant representatives of this species are Staphylococcus epidermidis and Staphylococcus aureus which grow predominantly as surface-attached stable communities known as biofilms. Cells in these biofilms are often highly resistant to antibiotics. Therefore, new antimicrobial or antibiofilm substances are still being developed. In this study, we investigated the influence of cobalt bis(dicarbollide) or its amino derivatives, and antibiotics (erythromycin, tetracycline)-alone and in combination-to determine the antimicrobial activity and the inhibition of biofilm formation against three type strains of S. epidermidis. We used a microcultivation device to determine the minimum inhibitory concentrations and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay to determine the metabolic activity of the cells in biofilms. We observed the synergistic effect of cobalt bis(dicarbollide) and its amino derivatives with both the antibiotics on both the planktonic and biofilm cells.
Permanent Link: http://hdl.handle.net/11104/0295557