Encyclopedia of Analytical Science

0503725 - ÚIACH 2020 RIV US eng M - Monography Chapter
Foret, František - Klepárník, Karel - Minárik, M.
Nucleic Acids | Chromatographic and Electrophoretic Methods.
Encyclopedia of Analytical Science. Vol. Third Edition. 3rd ed. Cambridge: Elsevier Ltd., 2019 - (Worsfold, P.; Townshend, A.; Poole, C.; Miró, M.), s. 1-9. vol. 8. ISBN 9780081019832
Institutional support: RVO:68081715
Keywords : electrophoresis * chromatographic methods * DNA separations
OECD category: Analytical chemistry

Techniques for separation of DNA and RNA provide powerful tools for the analysis of genetic material and transcription products.
Modern methods of analysis can answer most of the questions about the sequence, nucleotide substitutions (mutations, polymorphisms,
or single nucleotide polymorphismsdSNPs), and structure of nucleic acids. Crucial techniques in the analysis of DNA or
RNA are the traditional separation methodsdchromatography and electrophoresisdand, more recently, microfluidics and chipbased
DNA arrays and mass spectrometric methods. Chromatographic methods are commonly used in a variety of single-use kits for the isolation of genomic DNA from debris in
a cell lysate. Electrophoresis, on the other hand, plays an essential role in the analysis of the structure, sequence, and function of
DNA and RNA because of the high resolution achievable. Recently, however, new protocols were developed for chromatographic
DNA separations suitable also for analytical separations, especially for fast and highly efficient analysis of DNA mutations.
Permanent Link: http://hdl.handle.net/11104/0295517