Advances in the cellular structural biology of nucleic acids

0502737 - BFÚ 2019 RIV GB eng J - Journal Article
Giassa, I.C. - Ryneš, J. - Fessl, T. - Foldynová-Trantírková, Silvie - Trantírek, L.
Advances in the cellular structural biology of nucleic acids.
FEBS Letters. Roč. 592, č. 12 (2018), s. 1997-2011. ISSN 0014-5793. E-ISSN 1873-3468
R&D Projects: GA MŠMT EF15_003/0000477
Institutional support: RVO:68081707
Keywords : g-quadruplex structures * living mammalian-cells * human telomeric dna * resonance energy-transfer
OECD category: Biochemistry and molecular biology
Impact factor: 2.675, year: 2018
Method of publishing: Open access
https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.13054

Conventional biophysical and chemical biology approaches for delineating relationships between the structure and biological function of nucleic acids (NAs) abstract NAs from their native biological context. However, cumulative experimental observations have revealed that the structure, dynamics and interactions of NAs might be strongly influenced by a broad spectrum of specific and nonspecific physical-chemical environmental factors. This consideration has recently sparked interest in the development of novel tools for structural characterization of NAs in the native cellular context. Here, we review the individual methods currently being employed for structural characterization of NA structure in a native cellular environment with a focus on recent advances and developments in the emerging fields of in-cell NMR and electron paramagnetic resonance spectroscopy and in-cell single-molecule FRET of NAs.
Permanent Link: http://hdl.handle.net/11104/0294636