Megahertz serial crystallography

0501617 - FZÚ 2019 RIV GB eng J - Journal Article
Wiedorn, M.O. - Oberthuer, D. - Bean, R. - Andreasson, Jakob - Hajdu, Janos … Total 126 authors
Megahertz serial crystallography.
Nature Communications. Roč. 9, Oct (2018), s. 1-11, č. článku 4025. E-ISSN 2041-1723
R&D Projects: GA MŠMT EF16_019/0000789; GA MŠMT EF15_003/0000447
Grant - others:OP VVV - ADONIS(XE) CZ.02.1.01/0.0/0.0/16_019/0000789; OP VVV - ELIBIO(XE) CZ.02.1.01/0.0/0.0/15_003/0000447
Institutional support: RVO:68378271
Keywords : Free-Electron Laser * X-ray laser * m beta-lactamases * structure refinement * diffraction data * protein * radiation * software * pulses * PHENIX
OECD category: Fluids and plasma physics (including surface physics)
Impact factor: 11.878, year: 2018

The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a beta-lactamase from K. pneumoniae involved in antibiotic resistance.
Permanent Link: http://hdl.handle.net/11104/0293607