New Insight into Sperm Capacitation: A Novel Mechanism of 17 beta-Estradiol Signalling

0501577 - BTÚ 2019 RIV CH eng J - Journal Article
Bosakova, T. - Tockstein, A. - Šebková, Nataša - Šimoník, Ondřej - Adamusová, H. - Albrechtová, Jana - Albrecht, Tomáš - Bosáková, Z. - Dvořáková-Hortová, Kateřina
New Insight into Sperm Capacitation: A Novel Mechanism of 17 beta-Estradiol Signalling.
International Journal of Molecular Sciences. Roč. 19, č. 12 (2018), č. článku 4011. E-ISSN 1422-0067
R&D Projects: GA ČR(CZ) GA18-11275S; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:86652036 ; RVO:68081766
Keywords : 17 beta-estradiol * sperm * capacitation
OECD category: Biochemistry and molecular biology; Biochemistry and molecular biology (UBO-W)
Impact factor: 4.183, year: 2018

17 beta-estradiol (estradiol) is a natural estrogen regulating reproduction including sperm and egg development, sperm maturation-called capacitation-and sperm-egg communication. High doses can increase germ cell apoptosis and decrease sperm count. Our aim was to answer the biological relevance of estradiol in sperm capacitation and its effect on motility and acrosome reaction to quantify its interaction with estrogen receptors and propose a model of estradiol action during capacitation using kinetic analysis. Estradiol increased protein tyrosine phosphorylation, elevated rate of spontaneous acrosome reaction, and altered motility parameters measured Hamilton-Thorne Computer Assisted Semen Analyzer (CASA) in capacitating sperm. To monitor time and concentration dependent binding dynamics of extracellular estradiol, high-performance liquid chromatography with tandem mass spectrometry was used to measure sperm response and data was subjected to kinetic analysis. The kinetic model of estradiol action during sperm maturation shows that estradiol adsorption onto a plasma membrane surface is controlled by Langmuir isotherm. After, when estradiol passes into the cytoplasm, it forms an unstable adduct with cytoplasmic receptors, which display a signalling autocatalytic pattern. This autocatalytic reaction suggests crosstalk between receptor and non-receptor pathways utilized by sperm prior to fertilization.
Permanent Link: http://hdl.handle.net/11104/0293578