Multispectral atomic force microscopy-infrared nano-imaging of malaria infected red blood cells

0501540 - FZÚ 2019 RIV US eng J - Journal Article
Perez-Guaita, D. - Kochan, K. - Batty, M. - Doerig, C. - Garcia-Bustos, J. - Espinoza Herrera, Shirly J. - McNaughton, D. - Heraud, P. - Wood, Bayden R.
Multispectral atomic force microscopy-infrared nano-imaging of malaria infected red blood cells.
Analytical Chemistry. Roč. 90, č. 5 (2018), s. 3140-3148. ISSN 0003-2700. E-ISSN 1520-6882
R&D Projects: GA MŠMT EF15_003/0000447; GA MŠMT LQ1606
Grant - others:OP VVV - ELIBIO(XE) CZ.02.1.01/0.0/0.0/15_003/0000447
Institutional support: RVO:68378271
Keywords : IR spectroscopy * structural lipids * AFM-IR * hemozoin * proteins * quantification * localization * conformation * resolution * parasite
OECD category: Fluids and plasma physics (including surface physics)
Impact factor: 6.350, year: 2018

Atomic force microscopy-infrared (AFM-IR) spectroscopy is a powerful new technique that can be applied to study molecular composition of cells and tissues at the nanoscale. AFM-IR maps are acquired using a single wavenumber value: they show either the absorbance plotted against a single wavenumber value or a ratio of two absorbance values. Here, we implement multivariate image analysis to generate multivariate AFM-IR maps and use this approach to resolve subcellular structural information in red blood cells infected with Plasmodium falciparum at different stages of development. This was achieved by converting the discrete spectral points into a multispectral line spectrum prior to multivariate image reconstruction. The approach was used to generate compositional maps of subcellular structures in the parasites, including the food vacuole, lipid inclusions, and the nucleus, on the basis of the intensity of hemozoin, hemoglobin, lipid, and DNA IR marker bands, respectively.
Permanent Link: http://hdl.handle.net/11104/0293555