Cryo-SEM of Cell Cortex Architecture and Membrane Ultrastructure in Eugregarines

0501298 - ÚPT 2019 CZ eng A - Abstract
Vaškovicová, Naděžda - Kováčiková, M. - Valigurová, A. - Diakin, A. - Krzyžánek, Vladislav
Cryo-SEM of Cell Cortex Architecture and Membrane Ultrastructure in Eugregarines.
Mikroskopie 2018. Praha: Československá mikroskopická společnost, 2018. s. 76.
[Mikroskopie 2018. 14.05.2018-16.05.2018, Lednice na Moravě]
R&D Projects: GA ČR GA17-15451S; GA MŠMT(CZ) LO1212; GA MŠMT ED0017/01/01
Institutional support: RVO:68081731
Keywords : Cryo-SEM * Eugregarines * freeze-fracture * filamentous structures * cytoplasmic organelles
OECD category: Electrical and electronic engineering

Cryo-SEM of frozen microscopic structures, especially unicellular organisms, is a fast method for processing the sample for electron microscopy. Cells suspension in buffer were frozen in the high-pressure-freezing device HPM 100 (Leica microsystems) on 3 mm carriers and placed inside the cryo-high-vacuum chamber ACE 600 (Leica microsystems). Cells were fractured and after a short etching transferred into the SEM Magellan 400 (FEI) by vacuum shuttle VCT100 (Leica microsystems). The cryo-SEM analysis was performed at temperature of -120°C, electron probe current 3.1-6.3pA, and electron energy 1-2keV.
Permanent Link: http://hdl.handle.net/11104/0293290