Improving quality of optical fiber-based endoscopy for in vivo brain imaging

0501208 - ÚPT 2019 US eng A - Abstract
Tučková, Tereza - Hons, Miroslav - Jákl, Petr - Šiler, Martin - Dražanová, Eva - Krátká, Lucie - Traegaardh, Johanna - Pikálek, Tomáš - Simpson, Stephen Hugh - Tyc, Tomáš - Uhlířová, Hana - Čižmár, Tomáš
Improving quality of optical fiber-based endoscopy for in vivo brain imaging.
Neuroscience v2018. Book of Abstracts. Washington: SFN, 2018.
[Neuroscience 2018. 03.11.2018-07.11.2018, San Diego]
R&D Projects: GA MŠMT EF15_003/0000476
Institutional support: RVO:68081731

Optical fibers have been used in the past to image in locations deep in the scattering tissue which are not accessible through optical microscopy. Recently, the endeavor to minimize tissue damage has motivated the use of multi-mode fibers which allowed for high-resolution imaging with significantly smaller cross-section than single-mode fibers or GRIN lenses. Only recently the first successful functional imaging of neuronal calcium in vivo has been demonstrated in the mouse brain. These efforts represent a huge leap forward in MMF-based endoscopy, yet the quality of the detected functional calcium signals in vivo is still far from the state-of-the-art standards set by confocal and multi-photon microscopy. We introduce here computational and technological advancements to improve the quality of fiber-based imaging potentially important for brain endoscopy.
Permanent Link: http://hdl.handle.net/11104/0293201