Radiolabeling of the antibody IgG M75 for epitope of human carbonic anhydrase IX by Cu-61 and Cu-64 and its biological testing

0501136 - ÚJF 2020 RIV GB eng J - Journal Article
Čepa, Adam - Ráliš, Jan - Marešová, L. - Kleinová, M. - Seifert, Daniel - Sieglová, Irena - Král, Vlastimil - Polášek, Miloslav - Paurová, M. - Lázníček, M. - Lebeda, Ondřej
Radiolabeling of the antibody IgG M75 for epitope of human carbonic anhydrase IX by Cu-61 and Cu-64 and its biological testing.
Applied Radiation and Isotopes. Roč. 143, č. 1 (2019), s. 87-97. ISSN 0969-8043
R&D Projects: GA TA ČR TA02010797; GA MŠMT LM2015056
Institutional support: RVO:68378050 ; RVO:61389005
Keywords : Cu-64 * Cu-61 * phosphinate * Carbonic anhydrase IX * Tumour diagnostics * PET
OECD category: Radiology, nuclear medicine and medical imaging; Biochemistry and molecular biology (UMG-J)
Impact factor: 1.270, year: 2019
Method of publishing: Limited access
https://doi.org/10.1016/j.apradiso.2018.10.021

Human carbonic anhydrase IX is a membrane enzyme that is significantly expressed in some types of cancer cells, while copper radioisotopes offer wide range of diagnostic, therapeutic and theranostic properties. The work was focused on a new approach to the labelling of antibody IgG M75 for epitope human carbonic anhydrase IX with copper radioisotopes Cu-61 and Cu-64 and its in vivo testing in mice with inoculated colorectal cancer. Monoclonal antibody IgG M75 for epitope human carbonic anhydrase IX was successfully conjugated with copper-specific chelator 'phosphinate' and labelled with Cu-61 and Cu-64 The obtained molecule has considerable potential as a radioimmuno pharmaceutical suitable for imaging of tumours expressing carbonic anhydrase IX by positron emission tomography (PET).
Permanent Link: http://hdl.handle.net/11104/0293180