Capillary electrophoresis with preparative isoelectric focusing preconcentration for sensitive determination of amphotericin B in human blood serum

0501010 - ÚIACH 2020 RIV NL eng J - Journal Article
Šalplachta, Jiří - Horká, Marie - Šlais, Karel
Capillary electrophoresis with preparative isoelectric focusing preconcentration for sensitive determination of amphotericin B in human blood serum.
Analytica Chimica Acta. Roč. 1053, APR (2019), s. 162-168. ISSN 0003-2670. E-ISSN 1873-4324
R&D Projects: GA ČR(CZ) GA16-03749S; GA MZd(CZ) NV16-29916A; GA MV VI20172020069
Institutional support: RVO:68081715
Keywords : capillary zone electrophoresis * Amphotericin B * blood * sample preparation
OECD category: Analytical chemistry
Impact factor: 5.977, year: 2019
Method of publishing: Limited access

Amphotericin B (AmB) is still, despite its severe nephrotoxicity, the first-line agent in the management of serious systemic fungal infections. A sensitive and reliable method is therefore required to control AmB concentration in body fluids of a patient. This study demonstrates the potential of the off-line combination of preparative isoelectric focusing (IEF) with capillary isoelectric focusing (CIEF) or capillary zone electrophoresis (CZE) in the determination of AmB in human blood serum. The required value of the isoelectric point of AmB was determined to be 6.1 using the CIEF technique. Preparative IEF served as a pre-separation and concentration technique. The pH gradient was traced by colored low molecular pI markers. The collected fraction with AmB was easily processed and then analyzed by CIEF and CZE. Tens of picograms of AmB in human blood serum sample can be determined by a combination of preparative IEF with CZE. The method was linear in the AmB concentration range of 0.3e600 ngmL 1. The recovery ranged from 93% to 98%.
Permanent Link: http://hdl.handle.net/11104/0293037