Critical defects in cryopreserved cell nuclei: DNA structure changes

0500597 - FZÚ 2019 RIV CZ eng A - Abstract
Falk, Martin - Falková, Iva - Pagáčová, Eva - Kopečná, Olga - Bačíková, Alena - Šimek, Daniel - Golan, Martin - Follett, S.E. - Varga, K. - Teplá, O. - Kratochvílová, Irena
Critical defects in cryopreserved cell nuclei: DNA structure changes.
Materials Structure. Roč. 25, č. 1 (2018), s. 5-5. ISSN 1210-8529.
[XV Discussions in Structural Molecular Biology. 22.03.2019-24.03.2019, Nové Hrady]
Institutional support: RVO:68378271 ; RVO:68081707
Keywords : cell cryopreservation * DNA defects of cryopreserved cells * chromatin condensation * DMSO
OECD category: Biophysics
http://www.xray.cz/setkani/abst2018/falk.htm

For the first time, we described replicating cell-specific DNA damage and higher-order chromatin alterations after freezing/thawing. We simultaneously evaluated DNA defects and higher-order chromatin structure of frozen and thawed cells with and without cryoprotectant treatment. We found that in replicating (S phase) cells, DNA was preferentially damaged by replication fork collapse, potentially leading to DNA double strand breaks (DSBs), which represent an important source of both genome instability and defects in epigenome maintenance. Both in replicating and non-replicating cells, freezing and thawing altered the chromatin structure in a cryoprotectant-dependent manner.
Permanent Link: http://hdl.handle.net/11104/0292682