Human fibroblast post-thaw regeneration monitored by AFM and fluorescence microscopy

0500586 - FZÚ 2019 RIV US eng A - Abstract
Golan, Martin - Přibyl, J. - Pešl, M. - Jelínková, Š. - Acimovic, I. - Jaroš, J. - Rotrekl, V. - Falk, Martin - Skládal, P. - Kratochvílová, Irena
Human fibroblast post-thaw regeneration monitored by AFM and fluorescence microscopy.
Cryobiology. Elsevier. Roč. 85, č. 12 (2018), s. 133-134. ISSN 0011-2240. E-ISSN 1090-2392.
[CRYO 2018 (55th Annual Meeting of the Society for Cryobiology). 10.07.2018-13.07.2018, Madrid]
R&D Projects: GA MŠMT EF16_013/0001406; GA MŠMT(CZ) LO1409
Grant - others:OP VVV - SAFMAT(XE) CZ.02.1.01/0.0/0.0/16_013/0001406
Institutional support: RVO:68378271 ; RVO:68081707
Keywords : cryopreservation * cell stiffness * AFM * fluorescence microscopy * DMSO * PEG
OECD category: Biophysics

Cryopreservation of cells (mouse embryonic fibroblasts) is a fundamental task for wide range of applications. In this study by using AFM and fluorescence microscopy we showed how selected cryoprotectants (dimethyl sulfoxide and polyethylene glycol) affected the cryopreserved cells mechanical properties (stiffness) and how these parameters are correlated with cytoskeleton damage and reconstruction. We showed how cryopreserved (frozen and thawed) cells’ stiffness change according to type of applied cryoprotectant and its functionality in extracellular or intracellular space. We showed that AFM can be used as technique for investigation of cryopreserved cells surfaces state and development ex vivo. Our results offer a new perspective on the monitoring and characterization of frozen cells recovery by measuring changes in elastic properties by nanoindentation technique
Permanent Link: http://hdl.handle.net/11104/0292674