Macromolecular conjugates for isolation, immobilization and visualization of proteins

0500200 - ÚOCHB 2019 RIV eng P - Patent Document
Šácha, Pavel - Konvalinka, Jan - Schimer, Jiří - Knedlík, Tomáš - Šubr, Vladimír - Ulbrich, Karel - Strohalm, Jiří
Macromolecular conjugates for isolation, immobilization and visualization of proteins.
2018. Owner: Ústav organické chemie a biochemie AV ČR, v. v. i. - Ústav makromolekulární chemie AV ČR, v. v. i. - Univerzita Karlova v Praze. Date of the patent acceptance: 17.05.2018. Patent Number: AU2016207125
R&D Projects: GA ČR(CZ) GA16-02938S; GA MŠMT LO1302; GA MZd(CZ) NV15-31379A
Institutional support: RVO:61388963 ; RVO:61389013
Keywords : antibody mimetics * polymer * His tag
OECD category: Biochemistry and molecular biology; Polymer science (UMCH-V)
https://worldwide.espacenet.com/publicationDetails/originalDocument?FT=D&date=20180517&DB=&locale=en_EP&CC=AU&NR=2016207125B2&KC=B2&ND=4#

Macromolecular conjugates for isolation, immobilization and visualization of proteins The invention provides a synthetic macromolecular conjugate for selective interaction with proteins, comprising a synthetic copolymer, and at least one binding group and at least one further group selected from an affinity tag and an imaging probe, said at least one binding group and at least one further group being bound via covalent bond to said synthetic copolymer. The macromolecular conjugate is suitable in particular for identification, visualization, quantification or isolation of proteins and/ or cells. HPMA copolymer, i.e.poly(HPMA-co-Ma-β-Ala-TT), a copolymer prepared by conventional solution radical polymerization or controlled radical copolymerization (e.g. RAFT copolymerization, reversible addition-fragmentation chain-transfer) of N-(2- hydroxypropyl) methacrylamide (HPMA) and 3-(3-methacrylamidopropanoyl) thiazolidine-2-thione (Ma-P-Ala-TT) can be preferably used as the basic copolymer.
Permanent Link: http://hdl.handle.net/11104/0292995