Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber

0499922 - ÚPT 2019 RIV GB eng J - Journal Article
Vasquez-Lopez, S.A. - Turcotte, R. - Koren, V. - Ploschner, M. - Padamsey, Z. - Booth, M. - Čižmár, Tomáš - Emptage, N.J.
Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber.
Light-Science & Applications. Roč. 7, DEC (2018), č. článku 110. ISSN 2047-7538. E-ISSN 2047-7538
R&D Projects: GA MŠMT EF15_003/0000476
Institutional support: RVO:68081731
Keywords : light * microscopy * system * limits * mice
OECD category: Optics (including laser optics and quantum optics)
Impact factor: 14.000, year: 2018

Achieving intravital optical imaging with diffraction-limited spatial resolution of deep-brain structures represents an important step toward the goal of understanding the mammalian central nervous system(1-4). Advances in wavefront-shaping methods and computational power have recently allowed for a novel approach to high-resolution imaging, utilizing deterministic light propagation through optically complex media and, of particular importance for this work, multimode optical fibers (MMFs)(5-7). We report a compact and highly optimized approach for minimally invasive in vivo brain imaging applications. The volume of tissue lesion was reduced by more than 100-fold, while preserving diffraction-limited imaging performance utilizing wavefront control of light propagation through a single 50-mu m-core MMF. Here, we demonstrated high-resolution fluorescence imaging of subcellular neuronal structures, dendrites and synaptic specializations, in deep-brain regions of living mice, as well as monitored stimulus-driven functional Ca2+ responses. These results represent a major breakthrough in the compromise between high-resolution imaging and tissue damage, heralding new possibilities for deep-brain imaging in vivo.
Permanent Link: http://hdl.handle.net/11104/0292111