C-C bond cleavage in biosynthesis of 4-alkyl-L-proline precursors of lincomycin and anthramycin cannot precede C-methylation

0499534 - MBÚ 2019 RIV GB eng J - Journal Article
Kameník, Zdeněk - Gažák, Radek - Kadlčík, Stanislav - Steiningerová, Lucie - Rynd, Vít - Janata, Jiří
C-C bond cleavage in biosynthesis of 4-alkyl-L-proline precursors of lincomycin and anthramycin cannot precede C-methylation.
Nature Communications. Roč. 9, AUG 9 (2018), č. článku 3167. E-ISSN 2041-1723
R&D Projects: GA ČR(CZ) GJ17-13436Y
Institutional support: RVO:61388971
Keywords : Gene-cluster * Homolgy
OECD category: Microbiology
Impact factor: 11.878, year: 2018

The paper is a responding to the publication by Zhong et al.: 4-alkyl-L-(Dehydro) proline biosynthesis in actinobacteria involves N-terminal nucleophile-hydrolase activity of γ-glutamyltranspeptidase homolog for C-C bond cleavage. Nat. Commun. 8, 16109 (2017), in which the authors improperly deduced the order of reactions in the biosynthesis of 4-alkyl-L-proline derivative precursors of lincomycin and anthramycin. Based on the results of our research we documented the propper order of the reaction steps and wa also elucidated the mode of action of one of participating enzymes.
Permanent Link: http://hdl.handle.net/11104/0291730