Length computation of irradiated plasmid DNA molecules

0499497 - ÚJF 2019 RIV US eng J - Journal Article
Pachnerová Brabcová, Kateřina - Sihver, L. - Ukraintsev, Egor - Štěpán, Václav - Davídková, Marie
Length computation of irradiated plasmid DNA molecules.
Biointerphases. Roč. 13, č. 6 (2018), č. článku 061005. ISSN 1934-8630. E-ISSN 1559-4106
R&D Projects: GA ČR GJ17-03403Y
Institutional support: RVO:68378271 ; RVO:61389005
Keywords : atomic force microscopy * DNA fragments * irradiated DNA
OECD category: Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials; Health-related biotechnology (FZU-D)
Impact factor: 2.009, year: 2018

Compromised detection of short DNA fragments can result in underestimation of radiation-induced clustered DNA damage. The fragments can be detected with atomic force microscopy (AFM), followed by image analysis to compute the length of plasmid molecules. Plasmid molecules imaged with AFM are represented by open or closed curves, possibly with crossings. For the analysis of such objects, a dedicated algorithm was developed, and its usability was demonstrated on the AFM images of plasmid pBR322 irradiated with Co-60 gamma rays. The analysis of the set of the acquired AFM images revealed the presence of DNA fragments with lengths shorter than 300 base pairs that would have been neglected by a conventional detection method.
Permanent Link: http://hdl.handle.net/11104/0291688