A microfluidic device for investigation of cellular migration

0499343 - ÚIACH 2019 eng A - Abstract
Klepárník, Karel - Ledvina, Vojtěch
A microfluidic device for investigation of cellular migration.
Book of Abstracts. Single Cell Europe 2018. Vestec: BIOCEV, 2018. s. 76-76.
[Single Cell Europe Conference. 19.09.2018-21.09.2018, Praha]
R&D Projects: GA ČR(CZ) GA17-01995S
Institutional support: RVO:68081715
Keywords : microfluidic device * cancer cells * cell analysis
OECD category: Analytical chemistry

Functionality of a microfluidic device made of polydimethylsiloxane for testing epithelialmesenchymal transition of cancer cells is presented. Arrays of micropillars as obstacles
with different spacing are a prerequisite of high throughput measurements and standard evaluations of cellular deformability and migratory activity. The miniaturization
of the device with the total migration path length less than 1mm significantly shortened analysis time. The device is compatible with fluorescence microscope in a time-lapse setup
to visualize variability of mechanical properties of many individual cells under identical conditions. Cells were monitored for several hours.
Permanent Link: http://hdl.handle.net/11104/0291556