GENE EXPRESSION AND IMMUNOLOGICAL RESPONSE IN MICE EXPOSED TO ZnO NANOPARTICLES

0499201 - ÚEM 2019 RIV CZ eng C - Conference Paper (international conference)
Rössner ml., Pavel - Vrbová, Kristýna - Strapáčová, S. - Rössnerová, Andrea - Ambrož, Antonín - Brzicová, Táňa - Líbalová, Helena - Javorková, Eliška - Zajícová, Alena - Holáň, Vladimír - Kulich, P. - Večeřa, Zbyněk - Mikuška, Pavel - Coufalík, Pavel - Křůmal, Kamil - Čapka, Lukáš - Dočekal, Bohumil - Šerý, Omar - Machala, M. - Topinka, Jan
GENE EXPRESSION AND IMMUNOLOGICAL RESPONSE IN MICE EXPOSED TO ZnO NANOPARTICLES.
Nanocon 2017 : conference proceedings : 9th International Conference on Nanomaterials - Research & Application. Ostrava: Tanger Ltd., 2018, s. 650-655. ISBN 9788087294819.
[International Conference on Nanomaterials - Research and Application (NANOCON) /9./. Brno (CZ), 18.10.2017-20.10.2017]
R&D Projects: GA MŠMT(CZ) LO1508; GA ČR(CZ) GBP503/12/G147; GA MŠMT(CZ) LM2015073
Institutional support: RVO:68378041 ; RVO:68081715 ; RVO:67985904
Keywords : Zinc oxide nanoparticles * inhalation * gene expression * Immune response
OECD category: Genetics and heredity (medical genetics to be 3); Analytical chemistry (UIACH-O); Genetics and heredity (medical genetics to be 3) (UZFG-Y)

We analyzed gene expression changes in the lungs and the immunological response in splenocytes of mice exposed by inhalation of ZnO nanoparticles - NP. Adult female ICR mice were treated for three days and three months, respectively. Analysis of differential expression in genes involved in oxidative stress was conducted using quantitative RT-PCR. The potential immunotoxic and immunomodulatory effects of ZnO NP were analyzed by phenotyping and cytokine production by splenocytes after three months exposure. Three days exposure resulted in down-regulation of GCLC, GSR, HMOX-1, NQO-1, NF-kB2, PTGS2 and TXNRD1 mRNA expression, three months exposure increased the expression of these genes. Three months exposure caused a significant decrease in the percentage of granulocytes in the spleen cells, and affected the production of IL-10 and IL-6 by lipopolysaccharide-stimulated leukocytes. In summary, our study revealed changes in the expression of genes involved in the oxidative stress response following acute ZnO NP exposure. Subchronic ZnO NP exposure induced immunomodulatory effects in the spleen.
Permanent Link: http://hdl.handle.net/11104/0291448