Epitachophoretic separation and concentration of large volume samples

0499153 - ÚIACH 2019 RIV CZ eng C - Conference Paper (international conference)
Voráčová, Ivona - Foret, František - Datinská, Vladimíra - Novotný, Jakub - Gheibi, P. - Berka, J. - Astier, Y.
Epitachophoretic separation and concentration of large volume samples.
CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i., 2018 - (Foret, F.; Křenková, J.; Drobníková, I.; Klepárník, K.; Přikryl, J.), s. 316-319. ISBN 978-80-904959-5-1.
[CECE 2018. International Interdisciplinary Meeting on Bioanalysis /15./. Brno (CZ), 15.10.2018-17.10.2018]
R&D Projects: GA ČR(CZ) GBP206/12/G014
Institutional support: RVO:68081715
Keywords : epitachophoretic separation * DNA * large sample volumes
OECD category: Analytical chemistry
http://www.ce-ce.org/user_uploads/program/CECE%202018%20Proceedings_WOS.pdf

There has been a growing interest in developing isotachophoretic protocols for concentration and purification of DNA as an alternative to the solid phase extraction protocols of DNA preparation. Also concentration of DNA from large volume samples is required. We have developed a new device suitable for separation, concentration and collection of ions from several milliliter sample volumes into microliter fractions. We have used a discontinuous electrolyte system comprising of the leading and terminating electrolytes utilized for concentration of samples containing DNA fragments. The method is simple, fast with high concentration factor and without extensive surface interactions.
Permanent Link: http://hdl.handle.net/11104/0291419