A New evaluation of combined antitumor effects of natural and synthetic nuclear retinoid receptor ligands in human breast carcinoma cells

0499146 - ÚIACH 2019 RIV CZ eng C - Conference Paper (international conference)
Strouhalová, Dana - Laštovičková, Markéta - Bobál, P. - Macejová, D. - Mosná, B. - Brtko, J. - Bobálová, Janette
A New evaluation of combined antitumor effects of natural and synthetic nuclear retinoid receptor ligands in human breast carcinoma cells.
CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i., 2018 - (Foret, F.; Křenková, J.; Drobníková, I.; Klepárník, K.; Přikryl, J.), s. 283-285. ISBN 978-80-904959-5-1.
[CECE 2018. International Interdisciplinary Meeting on Bioanalysis /15./. Brno (CZ), 15.10.2018-17.10.2018]
Grant - others:AV ČR(CZ) SAV-18-16
Program: Bilaterální spolupráce
Institutional support: RVO:68081715
Keywords : nuclear retinoid X receptors * triorganotin compounds * breast carcinoma cells
OECD category: Analytical chemistry
http://www.ce-ce.org/user_uploads/program/CECE%202018%20Proceedings_WOS.pdf

This work represents the study of the effects of biologically active ligands of nuclear retinoid X receptors, triorganotin compounds, on proteomic profile of MDA-MB-231 cells. We focused primarily on tributyl/phenyltin derivatives that suggest the combined antitumor effect when they are used with natural nuclear retinoid receptor ligand all-trans retinoic acid (ATRA). Proteomic strategies connected to PDQuest evaluation were applied in this study. Some specific proteins with tumor process association were chosen for more detail study. Our findings indicate that selected triorganotins derivatives resulted to significantly reduced level of studied proteins belonging to metabolic pathway or to other cellular processes as apoptosis or epithelial–mesenchymal transition. In the case of vimentin, the key protein of EMT process, the silencing was set up by triorganotin compounds and significantly enhanced by combination with ATRA. This additive effect on downregulation can result from the potential engagement of RXR/RAR heterodimer, considering co-addition of both partner ligands of mentioned heterodimer to the treatment.
Permanent Link: http://hdl.handle.net/11104/0291415