Impact of cryopreservation on sterlet, Acipenser ruthenus sperm motility and proteome

0498849 - BC 2019 RIV NL eng J - Journal Article
Xin, M. - Shaliutina-Kolešová, A. - Štěrba, Ján - Koník, P. - Boryshpolets, S. - Rodina, M. - Li, P. - Nian, R. - Linhart, O.
Impact of cryopreservation on sterlet, Acipenser ruthenus sperm motility and proteome.
Animal Reproduction Science. Roč. 192, MAY (2018), s. 280-289. ISSN 0378-4320. E-ISSN 1873-2232
R&D Projects: GA MŠMT(CZ) EE2.3.30.0032; GA ČR GA15-03044S
Institutional support: RVO:60077344
Keywords : Cryodamage * Sperm proteins * Sperm quality
OECD category: Microbiology
Impact factor: 1.817, year: 2018

Fish sperm cryopreservation is a well-established technique allowing for artificial insemination on a commercial scale. The extent of proteome alterations in seminal plasma and sperm due to cryopreservation, however, is not known. This study was conducted to evaluate the effect of cryopreservation on motility variables of sterlet Acipenser ruthenus sperm and to detect the differences in protein profiles of fresh and cryopreserved sterlet sperm and seminal plasma. Fresh sperm had 89 ± 3% motility and 160 ± 14 μm/s curvilinear velocity at 15 s post-activation. The motility rate of cryopreserved sperm (37 ± 5%) was less at 15 s post-activation. No difference (ANOVA, P > 0.05) in mean curvilinear velocity of fresh and cryopreserved sperm was detected. The protein profiles of seminal plasma and sperm were characterized using comparative proteomics to determine the influence of cryopreservation. Six altered protein spots in seminal plasma and thirteen altered spots in sperm were detected in fresh and thawed sperm. Subsequent protein characterization suggested that the proteins identified were involved in sperm metabolism, cytoskeleton, and stress response. The results broaden the understanding of the effects of cryopreservation and identify the proteins associated with cryo-injury. These data may help to determine the function of altered proteins and provide new insights into improving sperm cryopreservation.
Permanent Link: http://hdl.handle.net/11104/0291139