Laser tweezers Raman spectroscopy of E. coli under antibiotic stress in microfluidic chips

0498740 - ÚPT 2019 RIV US eng C - Conference Paper (international conference)
Ježek, Jan - Pilát, Zdeněk - Bernatová, Silvie - Kirchhoff, F. - Tannert, A. - Neugebauer, U. - Samek, Ota - Zemánek, Pavel
Laser tweezers Raman spectroscopy of E. coli under antibiotic stress in microfluidic chips.
21st Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics. (Proceedings of SPIE 10976). Bellingham: SPIE, 2018 - (Zemánek, P.), č. článku 1097618. ISBN 9781510626072. ISSN 0277-786X.
[Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics /21./. Lednice (CZ), 03.09.2018-07.09.2018]
R&D Projects: GA ČR(CZ) GA16-07965S; GA MŠMT(CZ) LO1212; GA MŠMT ED0017/01/01
Institutional support: RVO:68081731
Keywords : Raman microspectroscopy * optical tweezers * optofluidics * E. coli - antibiotics
OECD category: Optics (including laser optics and quantum optics)

Analyzing the cells in various body fluids can greatly deepen the understanding of the mechanisms governing the cellular physiology. Because of the variability of physiological and metabolic states, it is important to be able to perform such studies on individual cells. Therefore, we developed an optofluidic system in which we precisely manipulated and monitored individual cells of Escherichia coli. We used laser tweezers Raman spectroscopy (LTRS) in a microchamber chip to manipulate and analyze individual E. coli cells. We subjected the cells to antibiotic cefotaxime, and we observed the changes by the time-lapse microscopy and Raman spectroscopy. We found observable changes in the cellular
morphology (cell elongation) and in Raman spectra, which were consistent with other recently published observations. We tested the capabilities of the optofluidic system and found it to be a reliable and versatile solution for this class of microbiological experiments.
Permanent Link: http://hdl.handle.net/11104/0291098