Combinatorial interplay of RNA-binding proteins tunes levels of mitochondrial mRNA in trypanosomes

0498061 - BC 2019 RIV US eng J - Journal Article
Dixit, Sameer - Lukeš, Julius
Combinatorial interplay of RNA-binding proteins tunes levels of mitochondrial mRNA in trypanosomes.
RNA. Roč. 24, č. 11 (2018), s. 1594-1606. ISSN 1355-8382. E-ISSN 1469-9001
R&D Projects: GA ČR GA15-21974S; GA ČR(CZ) GA16-18699S; GA MŠMT LL1601; GA MŠMT(CZ) EF16_019/0000759
Institutional support: RVO:60077344
Keywords : RNA editing * RNA binding proteins * ribonuclear protein * mitochondrion * trypanosome * iCLIP
OECD category: Biochemistry and molecular biology
Impact factor: 3.949, year: 2018

MRP1/2 is a heteromeric protein complex that functions in the trypanosomatid mitochondrion as part of the RNA editing machinery, which facilitates multiple targeted insertions and deletions of uridines. MRP1/2 was shown to interact with MRB8170, which initiates RNA editing by marking pre-edited mRNAs, while TbRGG2 is required for its efficient progression on pan-edited mRNAs. Both MRP1/2 and TbRGG2 are capable of modulating RNA-RNA interactions in vitro. As determined by using iCLIP and RIP-qPCR, RNAs bound to MRP1/2 are characterized and compared with those associated with MRB8170 and TbRGG2. We provide evidence that MRP1 and MRB8170 have correlated binding and similar RNA crosslinking peak profiles over minimally and never-edited mRNAs. Our results suggest that MRP1 assists MRB8170 in RNA editing on minimally edited mRNAs.
Permanent Link: http://hdl.handle.net/11104/0290526