Differential effects of the flavonolignans silybin, silychristin and 2,3-dehydrosilybin on mesocestoides vogae larvae (cestoda) under hypoxic and aerobic in vitro conditions

0497949 - MBÚ 2019 RIV CH eng J - Journal Article
Hrčková, G. - Kubašková Mačák, T. - Benada, Oldřich - Kofroňová, Olga - Tůmová, L. - Biedermann, David
Differential effects of the flavonolignans silybin, silychristin and 2,3-dehydrosilybin on mesocestoides vogae larvae (cestoda) under hypoxic and aerobic in vitro conditions.
Molecules. Roč. 23, č. 11 (2018), s. 2999. E-ISSN 1420-3049
R&D Projects: GA MŠMT(CZ) LTC18071; GA MŠMT(CZ) LO1509; GA ČR(CZ) GA18-00150S
Institutional support: RVO:61388971
Keywords : 2,3-dehydrosilybin * Aerobic and hypoxic cultivation * Mesocestoides vogae larvae
OECD category: Parasitology
Impact factor: 3.060, year: 2018

Mesocestoides vogae larvae represent a suitable model for evaluating the larvicidal potential of various compounds. In this study we investigated the in vitro effects of three natural flavonolignans—silybin (SB), 2,3-dehydrosilybin (DHSB) and silychristin (SCH)—on M. vogae larvae at concentrations of 5 and 50 µM under aerobic and hypoxic conditions for 72 h. With both kinds of treatment, the viability and motility of larvae remained unchanged, metabolic activity, neutral red uptake and concentrations of neutral lipids were reduced, in contrast with a significantly elevated glucose content. Incubation conditions modified the effects of individual FLs depending on their concentration. Under both sets of conditions, SB and SCH suppressed metabolic activity, the concentration of glucose, lipids and partially motility more at 50 µM, but neutral red uptake was elevated. DHSB exerted larvicidal activity and affected motility and neutral lipid concentrations differently depending on the cultivation conditions, whereas it decreased glucose concentration. DHSB at the 50 µM concentration caused irreversible morphological alterations along with damage to the microvillus surface of larvae, which was accompanied by unregulated neutral red uptake. In conclusion, SB and SCH suppressed mitochondrial functions and energy stores, inducing a physiological misbalance, whereas DHSB exhibited a direct larvicidal effect due to damage to the tegument and complete disruption of larval physiology and metabolism.
Permanent Link: http://hdl.handle.net/11104/0290399