Roughness of Transmembrane Helices Reduces Lipid Membrane Dynamics

0497636 - ÚFCH JH 2019 RIV US eng J - Journal Article
Olšinová, Marie - Jurkiewicz, Piotr - Kishko, Irina - Sýkora, Jan - Sabó, Ján - Hof, Martin - Cwiklik, Lukasz - Cebecauer, Marek
Roughness of Transmembrane Helices Reduces Lipid Membrane Dynamics.
iScience. Roč. 10, DEC 2018 (2018), s. 87-97. E-ISSN 2589-0042
R&D Projects: GA ČR GAP305/11/0459; GA ČR(CZ) GBP208/12/G016; GA ČR(CZ) GA18-26751S
Grant - others:GA MŠk(CZ) LM2015042
Institutional support: RVO:61388955
Keywords : LATERAL DIFFUSION * HYDROPHOBIC MISMATCH * PROTEIN INTERACTIONS
OECD category: Biophysics
Method of publishing: Open access

The dynamics of cellular membranes is primarily determined by lipid species forming a bilayer. Proteins are considered mainly as effector molecules of diverse cellular processes. In addition to large assemblies of proteins, which were found to influence properties of fluid membranes, biological membranes are densely populated by small, highly mobile proteins. However, little is known about the effect of such proteins on the dynamics of membranes. Using synthetic peptides, we demonstrate that transmembrane helices interfere with the mobility of membrane components by trapping lipid acyl chains on their rough surfaces. The effect is more pronounced in the presence of cholesterol, which segregates from the rough surface of helical peptides. This may contribute to the formation or stabilization of membrane heterogeneities. Since roughness is a general property of helical transmembrane segments, our results suggest that, independent of their size or cytoskeleton linkage, integral membrane proteins affect local membrane dynamics and organization.
Permanent Link: http://hdl.handle.net/11104/0290177