Cyanobacteria: From Basic Science to Applications

0497586 - ÚVGZ 2020 RIV GB eng M - Monography Chapter
Červený, Jan - Šalagovič, J. - Muzika, F. - Šafránek, D.
Influence of circadian clocks on optimal regime of central C-N metabolism of cyanobacteria.
Cyanobacteria: From Basic Science to Applications. London: Elsevier, 2019 - (Mishra, A.; Tiwari, D.; Rai, A.), s. 193-206. ISBN 978-0-12-814667-5
R&D Projects: GA ČR(CZ) GA15-17367S; GA MŠMT(CZ) LM2015055
Institutional support: RVO:86652079
Keywords : computational modeling * bioreactor * formal methods * industry 4.0 * microalgae * network analysis * optimization * process control
OECD category: Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology

Mechanistic aspects of cyanobacteria clock and metabolism have been analyzed in considerable detail during recent decades, but only limited knowledge is available about particular link(s) between the clock and controlled metabolic processes. We describe a case study where we couple a mechanistic model of a circadian oscillator with a model of central carbon-nitrogen metabolism and apply formal methods based on temporal logic and bifurcation analysis to examine response of the metabolic part of the combined model to the autonomous clock oscillations. The analysis identified a set of parametric groups that, when applied to the in silico system, lead either to an optimal configuration of metabolic pathways for most efficient rhythmic function of the metabolism or to (oscillatory) system death. Under controlled conditions in monitored environments, such as cultivation in photobioreactors, the knowledge can be used for optimal regulation of production capacity that would reflect dynamic constraints of real world systems.
Permanent Link: http://hdl.handle.net/11104/0290136