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Microfluidic device for cell counting and electrical lysis

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    0497535 - ÚIACH 2019 RIV CZ eng C - Conference Paper (international conference)
    Václavek, Tomáš - Křenková, Jana - Foret, František
    Microfluidic device for cell counting and electrical lysis.
    CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i., 2018 - (Foret, F.; Křenková, J.; Drobníková, I.; Klepárník, K.; Přikryl, J.), s. 63-68. ISBN 978-80-904959-5-1.
    [CECE 2018. International Interdisciplinary Meeting on Bioanalysis /15./. Brno (CZ), 15.10.2018-17.10.2018]
    R&D Projects: GA ČR(CZ) GJ16-09283Y; GA ČR(CZ) GBP206/12/G014
    Institutional support: RVO:68081715
    Keywords : microfluidic devices * microextraction of basic drugs * single cell proteomic analysis * electrospray ionization mass spectrometry * cell lysis
    OECD category: Analytical chemistry

    This lecture presents two microfluidic devices as essential units for single cell proteomic analysis, firstly a device for cell counting based on resistive pulse sensing as an alternative to optical platforms based on visual control over the sample input, secondly a unit for electrical lysis of cells using direct current voltage pulses to lyse cells one by one with a possibility to analyze the distinct lysate zones by electrospray ionization mass spectrometry.
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